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Quantitative proteomic analysis of UUO-14d kidneys from Adamts18+/+ versus Adamts18−/− mice using label-free LC-MS/MS

ABSTRACT: This project aims to investigate the proteomic differences between Adamts18+/+ and Adamts18−/− mice in response to unilateral ureteral obstruction (UUO) for 14 days. Kidney tissues were collected from both genotypes and subjected to quantitative proteomic analysis using label-free LC-MS/MS-based techniques. The workflow included protein extraction, trypsin digestion, peptide separation, and mass spectrometric analysis. Differentially expressed proteins were identified to explore the molecular mechanisms associated with Adamts18 deficiency in renal fibrosis under UUO conditions.

ORGANISM(S): Mus Musculus

SUBMITTER: Wei Zhang

PROVIDER: PXD064042 | iProX | Mon May 19 00:00:00 BST 2025

REPOSITORIES: iProX

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The secreted protease ADAMTS18 is a novel activator of latent TGF-β to exacerbate renal fibrosis.

Wang Min M Xu Fangmin F Niu Xiaohan X Zhang Mengxi M Liu Hanlin H Hong Tao T Dang Suying S Zhang Wei W

Communications biology 20250607 1

Renal fibrosis (RF) is an inevitable consequence of almost all forms of progressive chronic kidney disease (CKD). TGFβ is a powerful cytokine capable of dominating the fibrotic process. Targeting factors capable of activating latent TGF-β is a more effective and safe strategy to reduce TGF-β-induced fibrosis, but appropriate targets need to be identified. Here, we show that ADAMTS18/Adamts18 is significantly upregulated in the fibrotic kidneys of human CKD patients and mice. ADAMTS18 is primaril ...[more]

PMID: 40483302

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