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Proximity Profiling of Chloroplast–Endoplasmic Reticulum Membrane Contact Sites in Nicotiana benthamiana Using Split-TurboID During Viral Infection

ABSTRACT: We fused the N-terminus of TurboID to the chloroplast outer membrane protein OEP7 and the C-terminus to the endoplasmic reticulum membrane protein RTNLB2. When the chloroplast and endoplasmic reticulum come into close proximity to form membrane contact sites (MCSs), the split-TurboID is reconstituted into an active proximity-labeling enzyme that biotinylates nearby proteins. In this proteomic study, Barley stripe mosaic virus (BSMV) infection was used as the experimental variable.

ORGANISM(S): Nicotiana Benthamiana

SUBMITTER: Yongliang Zhang

PROVIDER: PXD064967 | iProX | Fri Jun 13 00:00:00 BST 2025

REPOSITORIES: iProX

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