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Data-independent acquisition (DIA) based quantitative proteomic analysis of dermal fibroblast supernatant samples

ABSTRACT: We collected fibroblasts derived from the foreskin of two healthy patients and cultured the cells in 7.5% HPL + DMEM medium. After in vitro expansion to passage 3 (P3), once the cells reached 80–90% confluency, the medium was replaced with an equal volume of fresh DMEM. The conditioned supernatant produced over a 3-day period was collected and subjected to proteomic sequencing to determine the protein composition of the fibroblast-derived supernatant.

ORGANISM(S): Homo Sapiens

SUBMITTER: An Qin

PROVIDER: PXD065596 | iProX | Fri Jun 27 00:00:00 GMT+01:00 2025

REPOSITORIES: iProX

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