Discovery of TRPV1-Associated Molecular Chaperones via PafA-Mediated Proximity Labeling

ABSTRACT: To identify TRPV1-associated molecular chaperones, we applied a PafA-based proximity labeling system using both N- and C-terminal TRPV1 fusions. Three groups were co-transfected with BCCP-Pup(E) for in-cell biotinylation. Biotinylated proteins were validated by immunofluorescence and Western blot, followed by streptavidin pull-down and mass spectrometry.

ORGANISM(S): Mus Musculus

SUBMITTER: Jianrong Xu  

PROVIDER: PXD066698 | iProX | Tue Jul 29 00:00:00 GMT+01:00 2025

REPOSITORIES: iProX