Project description:Impact of PPAR gamma overexpression and activation on rat pancreatic islet gene expression profile analyzed with oligonucleotide microarrays Keywords: parallel sample
Project description:Appendicular growth and bone mass acquisition are controlled by a variety of growth factors, hormones, and mechanical forces in a dynamic process called endochondral ossification. Chondrocytes in the growth plate must proliferate and undergo hypertrophy to drive growth plate expansion and lay the template for bone. Pleckstrin homology (PH) domain and leucine rich repeat phosphatase 1 and 2 (Phlpp1 and Phlpp2) are protein phosphatases that regulate intracellular signaling cascades through posttranslational modification of AKT, PKC, and S6K, among others. Phlpp1 controls chondrocyte proliferation and survival and germline deletion of Phlpp1 suppresses bone lengthening. Here, we demonstrate that Phlpp2 does not regulate endochondral ossification. Phlpp2-/- mice are phenotypically indistinguishable from their wildtype (WT) littermates, with similar bone length, bone mass, and growth plate dynamics. By contrast, Phlpp1/2-/- mice are phenotypically indistinguishable from Phlpp1-/- mice. Deletion of Phlpp1 or Phlpp2 had moderate effects on the chondrocyte transcriptome and proteome compared to WT control cells. By contrast, Phlpp1-/- and Phlpp1/2-/- chondrocytes had significantly altered phospho-proteomes compared to WT and Phlpp2-/- chondrocytes. Data integration via multiomics analysis revealed that RAF-MEK-ERK signaling was altered in cells lacking Phlpp1. In conclusion, these data demonstrate that Phlpp1, but not Phlpp2, regulates endochondral ossification via the chondrocyte phospho-proteome.
Project description:TRIM24 (Tripartite Motif-Containing 24) is a key transcriptional co-regulator involved in various cellular processes, including chromatin remodeling and gene expression. However, its role in cardiomyocytes remains largely unexplored. In this study, we investigated the global proteomic changes associated with TRIM24 overexpression in neonatal rat ventricular cardiomyocytes (NRVCMs) to better understand its function in cardiac biology. NRVCMs were transduced by an Adenovirus overexpressing TRIM24. Protein isolated from the NRVCMs transduced by an Adenovirus overexpressing LacZ was used as control.
Project description:Multiple rat microarrays were used to compare RNA transcript levels from 15,481 genes of baby rat kidney (BRK) cells transformed with the non-oncogenic Ad5 E1 or oncogenic Ad12 E1 region, as well as primary untransformed BRK cells.
Project description:To examine the effects of DPP4 overexpression and its modulation by 4,5-Dicaffeoylquinic acid on chondrocytes, we established C28/I2 cell lines with DPP4 overexpression (DPP4OE), control empty vector (OENC), and DPP4OE treated with 4,5-Dicaffeoylquinic acid. Gene expression profiling analysis was performed using RNA sequencing (RNA-seq) with biological replicates to investigate the global transcriptomic changes associated with these conditions.