Dataset Information

Proteomic analyses of protein biosynthesis in Landoltia punctata under biogas slurry stress.

ABSTRACT: This study focuses on the molecular mechanisms underlying the response of duckweed (Landoltia punctata) to high nitrogen and phosphorus stress in biogas slurry from pig farms. Through integrated transcriptomic and proteomic analyses, this study systematically elucidates, for the first time, how duckweed employs remarkable post-transcriptional regulatory strategies to remodel ribosomal function and photosynthetic carbon metabolism, thereby significantly enhancing protein synthesis and accumulation. The work provides novel insights into the adaptive mechanisms of aquatic plants under nutrient stress.

ORGANISM(S): Landoltia Punctata

SUBMITTER: Jiazhou Li

PROVIDER: PXD068264 | iProX | Wed Sep 10 00:00:00 BST 2025

REPOSITORIES: iProX

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Post-transcriptional regulation dominates protein biosynthesis in <i>Landoltia punctata</i> under biogas slurry stress.

Gong Xin-Li XL Li Jun-Yi JY Li Jia-Zhou JZ Ran Cheng C Zhou Le-Le LL Zhou Tian T Su Huan-Huan HH Lu Ting-Ting TT Zhang Shi-Lin SL

Frontiers in plant science 20251120

<h4>Introduction</h4>Duckweed represents a promising alternative protein source, yet enhancing its protein content remains essential for large-scale applications. This study investigated how high nitrogen and phosphorus stress from biogas slurry affects protein accumulation in Landoltia punctata and explored the underlying molecular regulatory mechanisms.<h4>Methods</h4><i>L. punctata</i> were cultivated in 1/5 strength Hoagland medium supplemented with 0-5% pig farm biogas slurry. The experimen ...[more]

PMID: 41358358

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Project description:The Chlamydomonas reinhardtii transcription factor PSR1 is required for the control of activities involved in scavenging phosphate from the environment during periods of phosphorus limitation. Increased scavenging activity reflects the development of high-affinity phosphate transport and the expression of extracellular phosphatases that can cleave phosphate from organic compounds in the environment. A comparison of gene expression patterns using microarray analyses and quantitative PCRs with wild-type and psr1 mutant cells deprived of phosphorus has revealed that PSR1 also controls genes encoding proteins with potential "electron valve" functions--these proteins can serve as alternative electron acceptors that help prevent photodamage caused by overexcitation of the photosynthetic electron transport system. In accordance with this finding, phosphorus-starved psr1 mutants die when subjected to elevated light intensities; at these intensities, the wild-type cells still exhibit rapid growth. Acclimation to phosphorus deprivation also involves a reduction in the levels of transcripts encoding proteins involved in photosynthesis and both cytoplasmic and chloroplast translation as well as an increase in the levels of transcripts encoding stress-associated chaperones and proteases. Surprisingly, phosphorus-deficient psr1 cells (but not wild-type cells) also display expression patterns associated with specific responses to sulfur deprivation, suggesting a hitherto unsuspected link between the signal transduction pathways involved in controlling phosphorus and sulfur starvation responses. Together, these results demonstrate that PSR1 is critical for the survival of cells under conditions of suboptimal phosphorus availability and that it plays a key role in controlling both scavenging responses and the ability of the cell to manage excess absorbed excitation energy. Set of arrays that are part of repeated experiments Biological Replicate Computed

Project description:Abiotic stress causes disturbances in the cellular homeostasis. Re-adjustment of balance in carbon, nitrogen and phosphorus metabolism therefore plays a central role in stress adaptation. However, it is currently unknown which parts of the primary cell metabolism follow common patterns under different stress conditions and which represent specific responses. To address these questions, changes in transcriptome, metabolome and ionome were analyzed in maize source leaves from plants suffering low temperature, low nitrogen (N) and low phosphorus (P) stress. The selection of maize as study object provided data directly from an important crop species and the so far underexplored C4 metabolism. Growth retardation was comparable under all tested stress conditions. The only primary metabolic pathway responding similar to all stresses was nitrate assimilation, which was down-regulated. The largest group of commonly regulated transcripts followed the expression pattern: down under low temperature and low N, but up under low P. Several members of this transcript cluster could be connected to P metabolism and correlated negatively to different phosphate concentration in the leaf tissue. Accumulation of starch under low temperature and low N stress, but decrease in starch levels under low under low P conditions indicated that only low P treated leaves suffered carbon starvation. In conclusion, maize employs very different strategies for management of nitrogen and phosphorus metabolism under stress. While nitrate assimilation was regulated depending on demand by growth processes, phosphate concentrations changed depending on availability, thus building up reserves under excess conditions. Carbon and energy metabolism of the C4 maize leaves were particularly sensitive to P starvation. Responses of maize source leaves to low temperature, low nitrogen and low phosphorus conditions were tested in independent single-stress experiments. Seedlings were cultivated in pots containing nutrient-poor peat soil under the controlled conditions of a growth chamber. The plants were fertilized with modified Hoagland solutions, containing 15mM KNO3 and 0.5mM KH2PO4 for control conditions; for low N and low P treatment, the nutrient concentrations were reduced to 0.15mM KNO3 and 0.1mM KH2PO4, respectively. Low temperature treated plants were always supplied with control nutrient solution. Plants from the nitrogen and phosphorus experiment as well as the control temperature plants were exposed to 28Â°C during the day and 20Â°C during the night. Low temperature treatment was limited to the night period and was reduced to 4Â°C for the 10h dark period. Source leaf lamina were harvested at day 20 (low temperature experiment) or day 30 after start of germination (low nitrogen and low phosphorus experiment) for parallel analysis of transcriptome, metabolome and ion profiles. The molecular data is further supplemented by phenotypic characterization of the maize seedlings under investigation.

Dataset Information

Proteomic analyses of protein biosynthesis in Landoltia punctata under biogas slurry stress.

Publications

Post-transcriptional regulation dominates protein biosynthesis in <i>Landoltia punctata</i> under biogas slurry stress.

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Dataset Information

Proteomic analyses of protein biosynthesis in Landoltia punctata under biogas slurry stress.

Publications

Post-transcriptional regulation dominates protein biosynthesis in &lt;i&gt;Landoltia punctata&lt;/i&gt; under biogas slurry stress.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Post-transcriptional regulation dominates protein biosynthesis in <i>Landoltia punctata</i> under biogas slurry stress.