Project description:To investigate the transcriptomic changes in the meninges located between olfactory bulb of adult and aged mice. Whole meningeal cells in adult and aged mice were FACS sorted and single-cell RNA seq was performed.
Project description:Currently, the mechanisms behind the anti-aging effects of exercise are not understood. The presented study conducted a microarray on hippocampal samples from adult (3.5 month-old) and aged (18 month-old) male BALB/c mice that were individually housed with or without running wheels for 8 weeks. Results showed that aging altered genes related to chromatin remodeling, cell growth, immune activity, and synapse organization compared to adult mice. Exercise was found to modulate many of the genes altered by aging, but in the opposite direction. For example, wheel running increased expression of genes related to cell growth and attenuated expression of genes involved in immune function and chromatin remodeling. Collectively, findings show that even late-onset exercise may attenuate age-related changes in gene expression and identifies possible pathways through which exercise may exert its beneficial effects. In total 20 samples (4 aged exercise, 4 aged sedentary, 6 adult exercise, and 6 adult sedentary) plus 4 technical replicates (24 total) were analyzed.
Project description:The goal of this study was to determine whether depletion and repopulation of microglia in adult and aged mice reversed age-related immune priming.
Project description:This laboratory studies the role of carbohydrates in the development of the central nervous system. Comparison of glycosyltransferase gene expression in developing rodent cerebellum with the aim of understanding the regulation of tissue-specific glycans RNA from adult and postnatal day 7 BALB/c mouse cerebellum was extracted and analyzed for differences in glycosyltransferase expression. Three replicate samples were processed for each age group and hybridized to the GLYCOv2 array.
Project description:Here we characterized and compared differential gene expression from diabetic and normoglycemic NOD mice and from aged and young-adults Balb/c mice. Total RNA from NOD diabetic (3 weeks with glicemia over 500mg/dl) and normoglycemic NOD mice from the same age was used. To compare the effects of aging in genomic expression, total RNA from young-adult Balb/c (9 weeks old) and middle-aged Balb/c (47 weeks old) were used.
Project description:10x Genomics Xenium in situ targeted gene expression was performed on FFPE mouse brain tissue using the Xenium Mouse Brain Gene Expression panel (mBrain_v1.1). The staged dataset contains adult and aged cohorts with control and heat-stress sample annotations. Heat-stress mice received periodic heat exposure at approximately 40 deg C for 3 hours/day for 14-15 days. Raw files include high-resolution morphology images and decoded transcripts; processed files include transcript count matrices, cell summaries, and cell and nucleus segmentation boundaries.
Project description:Mice deficient in the glucocorticoid-regenerating enzyme 11β-HSD1 resist age-related spatial memory impairment. To investigate the mechanisms/pathways involved, we used microarrays to identify differentially expressed hippocampal genes that associate with cognitive ageing and 11β-HSD1. Aged wild-type mice were separated into memory-impaired and unimpaired relative to young controls according to their performance in the Y-maze. All individual aged 11β-HSD1-deficient mice showed intact spatial memory. The majority of differentially expressed hippocampal genes were increased with ageing (e.g. immune/inflammatory response genes) with no genotype differences. However, the neuronal-specific transcription factor, Npas4 and immediate early gene, Arc were reduced (relative to young) in the hippocampus of memory-impaired but not unimpaired aged wild-type or aged 11β-HSD1-deficient mice. Quantitative RT-PCR and in situ hybridization confirmed reduced Npas4 and Arc mRNA expression in memory-impaired aged wild-type mice. These findings suggest that 11β-HSD1 may contribute to the decline in Npas4 and Arc mRNA levels associated with memory impairment during ageing, and that decreased activity of synaptic plasticity pathways involving Npas4 and Arc may, in part, underlie the memory deficits seen in cognitively-impaired aged wild-type mice. 20 samples, 5 groups of 4 biological replicates each. Young, Wild Type animals are overall controls