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Shigella flexneri evades septin-mediated cell-autonomous immunity via protein ADP-riboxanation

ABSTRACT: We demonstrate that OspC effectors enable S. flexneri to evade septin cage entrapment. Mechanistically, OspC catalyzes ADP-riboxanation of SEPT9 at Arg561, a critical site essential for stabilizing septin hetero-oligomers. Notably, ADP-riboxanation or mutation of Arg561 impairs septin polymerization and hence the assembly of higher-order structures, including filaments and cage-like structures. Finally, we provide evidence that S. flexneri OspC effectors act synergistically with OspG to antagonize septin cage entrapment via two distinct post-translational modifications.

ORGANISM(S): Homo Sapiens

SUBMITTER: Xiaoyun Liu

PROVIDER: PXD070327 | iProX | Tue Nov 04 00:00:00 GMT 2025

REPOSITORIES: iProX

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