Quantitative proteomic analysis reveals the inflammatory regulation and immune activation of mild magnetic hyperthermia in bladder cancer

ABSTRACT: Inflammatory responses and immune regulation in bladder cancer treatment are critical to therapeutic efficacy, yet their specific mechanisms and protein-level changes remain unclear. Previous studies have shown that an immunosuppressive tumor microenvironment and excessive neutrophil infiltration lead to poor therapeutic safety and efficacy. In this study, a mild magnetic hyperthermia (mMHT) protocol was employed to perform low-temperature tumor magnetic hyperthermia, successfully achieving tumor inhibition and inflammation modulation in a bladder cancer-bearing mouse model. Through proteomic analysis of tumor tissues from treated mice, the mechanisms of mMHT against bladder cancer were elucidated, particularly its roles in immune activation and inflammation suppression. mMHT induces significant global protein expression reprogramming, and principal component analysis (PCA) revealed clear distinctions among the five groups, indicating distinct proteomic profiles. Differential protein analysis showed that, compared with the control group (MB49a), the mMHT group (MB49e) exhibited 2,391 upregulated proteins and 3,133 downregulated proteins, with the most pronounced differences observed relative to the three single-treatment groups. Venn analysis identified 3,259 unique differential proteins in the mMHT group, highlighting the synergistic molecular response network triggered by the combination of magnetic hyperthermia and NO therapy.

ORGANISM(S): Mus Musculus

SUBMITTER: Jiawei Liu  

PROVIDER: PXD070387 | iProX | Wed Nov 05 00:00:00 GMT 2025

REPOSITORIES: iProX