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Characterization, Antioxidant Effects of Lycium ruthenicum Murr. Polysaccharides and its Protective Effects Against Oxaliplatin-Induced Peripheral Neurotoxicity by Inhibiting the RAS-RAF-MEK-ERK Signaling Pathway

ABSTRACT: Oxaliplatin is a frequently used chemotherapeutic agent; however, oxaliplatin-induced peripheral neuropathy (OIPN) is a prevalent adverse effect for which no effective treatment currently exists. Lycium ruthenicum Murr. (LR), a traditional Chinese medicinal herb also consumed as food, is rich in polysaccharides. However, the influence of alcohol precipitation concentration on the structure and bioactivities of polysaccharides from Lycium ruthenicum Murr (LRPs) remains inadequately understood, nor is its effect on OIPN. In this study, three novel LRPs (LRP-20, LRP-50, and LRP-80) were isolated using 20%, 50% and 80% ethanol, respectively, from crude LRPs extracted with hot water. LRP-20 (5846 Da), LRP-50 (7327 Da), and LRP-80 (9980 Da) differed in monosaccharide composition and content. FT-IR, SEM, XRD, and thermal stability assessments revealed distinct differences in the structural properties among the LRPs. In vitro tests found LRP-20 exhibited the highest antioxidant activity, relevant to OIPN's main pathogenic mechanisms. In OIPN rat models, LRP-20 potentially alleviated OIPN by augmenting antioxidant capacity and attenuating inflammatory responses mediated through the RAS-RAF-MEK-ERK signaling pathway. This research demonstrated that ethanol fractional precipitation can initially separate polysaccharides according to the structure, which exhibits strong correlation with the biological activities, and providing an approach for the further development of LPRs in the field of peripheral nerve protection.

ORGANISM(S): Rattus Norvegicus

SUBMITTER: Jianhua Yang

PROVIDER: PXD071288 | iProX | Wed Nov 26 00:00:00 GMT 2025

REPOSITORIES: iProX

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