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Identification of ubiquitination sites on the GSTM2 protein

ABSTRACT: The human GSTM2 protein was enriched, followed by mass spectrometry to identify its ubiquitination sites.

ORGANISM(S): Homo Sapiens

SUBMITTER: Junying Miao

PROVIDER: PXD071988 | iProX | Mon Dec 15 00:00:00 GMT 2025

REPOSITORIES: iProX

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Small molecule HCY-NBD stabilizes GSTM2 via cys174 sulfenylation to attenuate high glucose induced endothelial cell senescence and calcification.

Zhang Yangyang Y Dong Xinyu X Yan Xiaomeng X Zhao Congyao C Chi Xiaohui X Zhao Baoxiang B Miao Junying J Hong Fanzhen F Lin Zhaomin Z

Redox biology 20260127

Exogenous Glutathione S-transferase Mu 2 (GSTM2) supplementation has emerged as a promising strategy to counteract aging. However, approaches to enhance endogenous GSTM2 expression remain underexplored. Here, we identify HCY-NBD, an SO<sub>2</sub>-targeting small molecular that binds GSTM2 and stabilizes GSTM2 protein levels under high-glucose (HG)-induced vascular endothelial senescence. Mechanistically, HCY-NBD promotes sulfenylation at Cys174 of GSTM2 and inhibits its K48-linked ubiquitinatio ...[more]

PMID: 41637880

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