Nuclear polysaccharides maintain H3K9me3-heterochromatin and genomic stability

ABSTRACT: Polysaccharides are traditionally confined to the cell surface and extracellular space, whereas their nuclear roles remain unclear. Here, we show that N-glycans are present in the nucleus and modify inner nuclear membrane (INM) proteins across multiple cell types. Disruption of INM protein N-glycosylation reduces H3K9me3 in lamina-associated domains and induces genomic instability by impairing SETDB1 association with the INM. Notably, canonical ER N-glycan biosynthetic machinery contributes to INM protein N-glycosylation. Our findings reveal a previously unrecognized nuclear function of polysaccharides.

ORGANISM(S): Mus Musculus

SUBMITTER: Shisheng Sun  

PROVIDER: PXD072505 | iProX | Tue Dec 30 00:00:00 GMT 2025

REPOSITORIES: iProX