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Chaperone-mediated autophagy supports organ regeneration and fibroblast quiescence in mouse models of fibrosis

ABSTRACT: Chaperone-mediated autophagy (CMA) acting as a crucial, selective protein degradation pathway is a key topic in understanding tissue remodeling and disease, with recent research showing its pivotal role in maintaining organ health, particularly through the maintenance of fibroblast quiescence and supporting regeneration in fibrosis models.

ORGANISM(S): Mus Musculus

SUBMITTER: Jiazhen Wang

PROVIDER: PXD073386 | iProX | Thu Jan 22 00:00:00 GMT 2026

REPOSITORIES: iProX

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Project description:This SuperSeries is composed of the SubSeries listed below. Project abstract: In adult mammals, skin wound healing has evolved to favor rapid repair through the formation of fibrotic scar. These dermal scars are dysfunctional and may lead to chronic disfigurement and disability, yet the biologic mechanisms that drive fibrosis and prevent tissue regeneration remain unknown. Here, we report that reindeer (Rangifer tarandus) antler velvet exhibits regenerative wound healing, whereas identical full-thickness injury in dorsal back skin of the same animal forms fibrotic scar. This regenerative capacity is retained even following ectopic transplantation of velvet to a scar-forming site, demonstrating that this latent regenerative capacity is innate to velvet cells and independent of local factors derived from the growing antler. Single cell RNA-sequencing of uninjured skin revealed a marked divergence in resting fibroblast transcriptional states and immunomodulatory function. Uninjured velvet fibroblast shared a striking resemblance with human fetal fibroblasts whereas uninjured back skin fibroblasts exhibited an overrepresentation of pro-inflammatory genes resembling adult human fibroblasts. Identical skin injury resulted in site-specific fibroblast polarization; back fibroblasts exacerbated the inflammatory response, whereas velvet fibroblasts adopted an immunosuppressive state and reverted back to a regeneration-competent ground state. Consequently, velvet wounds exhibited an accelerated adoption of anti-inflammatory immune states and an expedited resolution of immune response. This study demonstrates reindeer as a novel comparative mammalian model to study both adult skin regeneration (velvet) and scar formation (back skin) within the same animal. Our study underscores the importance of fibroblast heterogeneity in shaping local immune cell functions that ultimately polarize wound healing outcomes. Purposeful, acute modulation of fibroblast-mediated immune signaling represents an important therapeutic avenue to mitigate scar and improve wound healing.

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Chaperone-mediated autophagy supports organ regeneration and fibroblast quiescence in mouse models of fibrosis

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