Cell Membrane Protein-centered Interactome Analysis Enabled by In-Vivo Cross-Linking Combined with Glycan-Directed Enrichment
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ABSTRACT: a biocompatible, mass spectrometry (MS)-cleavable crosslinker, trehalose disuccinimidyl ester (TDS), was used to “freeze” interactions between membrane proteins and their partners with enhanced cross-linking and identification capabilities. This was coupled with a glycan-directed enrichment strategy based on protein-level hydrazine chemistry, allowing efficient isolation of crosslinked cell membrane proteins interacting proteins for further analysis.
ORGANISM(S): Homo Sapiens
SUBMITTER:
Ci Wu
PROVIDER: PXD073835 | iProX | Fri Jan 30 00:00:00 GMT 2026
REPOSITORIES: iProX
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