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Cysteine-enabled Cleavability to Advance Cross-linking Mass Spectrometry for Global Analysis of Endogenous Protein-Protein Interactions

ABSTRACT: We have employed the sulfoxide-based cleavability to establish a novel cysteine-based MS-cleavable XL-MS platform, enabling proteome-wide PPI analysis using non-cleavable heterobifunctional K-C linkers. The effectiveness of the newly established platform has been demonstrated using three commercially available and non-cleavable NHS ester-haloacetamide heterobifunctional cross-linkers to map in vivo PPIs from HEK 293 cells.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Fenglong Jiao

LAB HEAD: Lan Huang

PROVIDER: PXD055169 | Pride | 2025-10-17

REPOSITORIES: Pride

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	20220212_01.txt	Txt
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	20220212_07.txt	Txt
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