Proteomics

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Proteomic analysis of extracellular vesicles from inflammatory fibroblasts in colorectal cancer lung metastasis

ABSTRACT: Pulmonary metastasis is a major cause of mortality in colorectal cancer (CRC), and the tumor microenvironment plays a critical role in metastatic progression. In particular, inflammatory fibroblasts have been implicated in shaping the metastatic niche; however, the molecular characteristics of their extracellular vesicles (EVs) remain incompletely understood. In this study, we performed LC-MS/MS-based proteomic analysis of EVs derived from IL-1β–stimulated MRC-5 lung fibroblasts. EVs were isolated from conditioned media of MRC-5 cells with or without IL-1β treatment and subjected to comparative proteomic profiling. This dataset provides a resource for characterizing EV-associated protein alterations in inflammatory fibroblasts and supports further investigation of their potential roles in tumor-related processes in colorectal cancer lung metastasis.

ORGANISM(S): Homo Sapiens

SUBMITTER: Weipeng Wang  

PROVIDER: PXD075933 | iProX | Fri Mar 20 00:00:00 GMT 2026

REPOSITORIES: iProX

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Vesicular QSOX1-MMP2 from inflammatory cancer-associated fibroblasts degrades the extracellular matrix to drive colorectal cancer pulmonary dissemination.

Ye Tingwei T   Chen Mengxi M   Hu Yu Y   Yang Ziyan Z   Xu Jiayu J   Zhang Yongjie Y   Zhou Shenyue S   Yu Yuanyuan Y   Chen Yinshuang Y   Zhang Zhoudong Z   Wang MengMeng M   Ruan Jianqing J   Zhang Weitao W   Zhang Haiyang H   Chen Weichang W   Wang Weipeng W  

Cancer letters 20260530

Pulmonary metastases represent a leading cause of mortality in colorectal cancer (CRC); however, the stromal mechanisms underlying metastatic colonization remain incompletely understood. Here, we show that extracellular vesicles (EVs) derived from inflammatory cancer-associated fibroblasts (iCAFs) are key promoters of CRC pulmonary metastasis (CRPM). Using single-cell RNA sequencing in a CRPM mouse model, we found that iCAFs constitute the predominant fibroblast population within the metastatic  ...[more]

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