Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genetic code expansion enables site-specific acetylation to reprogram Lunasin toward BRD4 targeting

ABSTRACT: Lunasin is a plant-derived peptide with moderate anticancer activity but has limited potency and undefined molecular targets. To enhance its functional properties, we developed a site-specifically acetylated variant (Lunasin-K29ac) via genetic code expansion. To characterize the molecular interactions underlying its enhanced activity, we performed interactome profiling of a control (pET-22b-CBD), wild-type Lunasin, and Lunasin-K29ac in HT-29 cells using His pull-down MS.

ORGANISM(S): Homo Sapiens

SUBMITTER: Nanxi Wang

PROVIDER: PXD076719 | iProX | Tue Apr 07 00:00:00 BST 2026

REPOSITORIES: iProX

ACCESS DATA

Json Xml

Similar Datasets

Genetic code expansion reveals site-specific lactylation in living cells reshapes protein function

Project description:Genetic code expansion reveals site-specific lactylation in living cells reshapes protein function

2023-12-26 | PXD048127 |

Genetic code expansion reveals aminoacylated lysine ubiquitination mediated by UBE2W

Project description:Protein post-translational modification regulates near every aspect of cellular processes in eukaryotes. However, the identification of new protein post-translational modification is very challenging. Here, using genetically encoded unnatural amino acids as chemical probes, we report the identification and validation of a previously unreported form of protein post-translational modification, aminoacylated lysine ubiquitination, in which the modification occurs on the α-amine group of aminoacylated lysine. We identify more than two thousands of unique aminoacylated lysine ubiquitination sites on all 20 aminoacylated lysines in two human cell lines. The aminoacylated lysine ubiquitination modifications can mediate rapid protein degradation, complementing the canonical lysine ubiquitination-mediated proteome degradation. Furthermore, we demonstrate that the ubiquitin conjugating enzyme UBE2W acts as a writer of aminoacylated lysine ubiquitination and facilitates the ubiquitination of internal α-amine groups on proteins using an unexpected enzymatic activity. More broadly, the discovery and validation of aminoacylated lysine ubiquitination paves the way for the identification and verification of new protein post-translation modifications with the genetic code expansion strategy.

2022-07-20 | PXD035166 |

Expression data of colorectal cancer HT-29 cells

Project description:Colorectal cancer HT-29 cell line is a comonly-used human cancer cell line. We have used this cell line for examining the effect of various anticancer compounds on gene expression and we obtained gene expression data of untreated HT-29 cells as a control data for the analysis.

2017-06-14 | GSE99968 | GEO

Genetic code expansion reveals site-specific lactylation in living cells reshapes protein function

Project description:Still in its infancy, the functions of lactylation remain elusive. To address this, we established a comprehensive workflow for lactylation studies that integrates the discovery of lactylation sites with proteomics, the expression of site-specifically lactylated proteins in living cells via genetic code expansion (GCE), and the evaluation of the resulting biological consequences. Specifically, we developed a wet-and-dry-lab combined proteomics strategy, and identified highly conserved lactylation at ALDOA-K147. Driven by its potential biological significance, we site-specifically expressed this lactylated ALDOA in mammalian cells and interrogated the biological changes. We discovered that it not only inhibited enzyme activity but also elicited gain-of-function effects——it dramatically reshaped the functionality of ALDOA by improving stability, enhancing nuclear translocation and affecting gene expression. Further, we demonstrated broad applicability of this workflow to study distinct histone lactylation sites. Together, we anticipate its wide uses in elucidating causative links between site-specific lactylation and target-centric or cell-wide changes.

2024-02-12 | GSE252184 | GEO

Genetic Code Expansion in Animals.

Project description: Not available

| S-EPMC7026698 | biostudies-literature

LC-MS/MS Genetic code expansion(GCE)

Project description:#1. Bottom-up data of ALDOA-K147 purified protein ALDOA-K147-purified-protein #2. Bottom-up data of IP-MS analysis of Klac and KlacOEt 20231016-DIA-IP-N1-con 20231016-DIA-IP-N2-con 20231016-DIA-IP-N3-con 20231016-DIA-IP-N1-Klac 20231016-DIA-IP-N2-Klac 20231016-DIA-IP-N3-Klac 20231016-DIA-IP-N1-KlacOEt 20231016-DIA-IP-N2-KlacOEt 20231016-DIA-IP-N3-KlacOEt 20231016-DDA-IP-con 20231016-DDA-IP-Klac 20231016-DDA-IP-KlacOEt 20231016-input-con 20231016-input-Klac 20231016-input-KlacOEt #3. TRAP analysis of ALDOA-WT and ALDOA-K147 20240307-TRAP-A1 20240307-TRAP-A2 20240307-TRAP-A3 20240307-TRAP-A4 20240307-TRAP-K1 20240307-TRAP-K2 20240307-TRAP-K3 20240307-TRAP-K4

2024-12-15 | PXD052776 | Pride

Inducible Genetic Code Expansion in Eukaryotes.

Project description: Not available

| S-EPMC7754456 | biostudies-literature

Whole Exome Sequencing Data of Dabrafenib Resistant HT-29 Cell Line

Project description:HT-29 cells were barcoded using the CloneTracker lentiviral barcode library and then dabrafenib resistant derivatives of these cell lines were established, respectively. Five million barcoded HT-29 cells were seeded into 15 cm cell culture dishes. When the cells reached confluency, two million cells per dish were seeded into four different 15 cm dishes (DMSO Control, Replica A, B, C) and two million cell pellets were stocked as initial cell population. Harvesting used medium through the experiment was performed at monthly intervals. Barcoded HT-29 cell line replicates A, B, and C were treated with 2XIC50 (199.6 nM) of dabrafenib concentration for the duration of 3 months.Barcoded data can be accessed via accession code E-MTAB-13018. Whole exome sequencing of dabrafenib-resistant A replicate and DMSO control cell lines were carried out.

2023-09-06 | E-MTAB-13338 | biostudies-arrayexpress

The Genetic Architecture of Congenital Diarrhea and Enteropathy (CODE)

Project description:Next-generation sequencing (NGS) has supported precision therapeutic approaches that have improved the lives of children with rare diseases. Patients with congenital diarrhea and enteropathies (CODE) have a particularly difficult disease with high morbidity and mortality. However, there are now several targeted therapies including specific diets, pharmacological treatments, and surgical interventions that are based on an individual’s genetic diagnosis. We performed NGS on a large cohort of 139 infants with suspected monogenic congenital diarrheal disorders and identified known causal variants in 50% of cases, including a new founder NEUROG3 variant. We also uncovered and functionally characterized three novel CODE genes, GRWD1, MYO1A, and MON1A, using cell and zebrafish models.

2025-05-21 | GSE264461 | GEO

Genetic code expansion in the mouse brain.

Project description: Not available

| S-EPMC5215917 | biostudies-literature