Proteomics

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Identification of regulatory protein networks of SEC14L2 in 293T cells


ABSTRACT: For mass spectrometry analysis of SEC14L2 and STMN1 binding partners, 293T cells expressing SEC14L2-Flag or STMN1-Flag were lysed in NP-40 lysis buffer. The cytosolic proteins were collected by centrifugation and incubated with anti-Flag beads for 2 h, followed by five washes with NP-40 lysis buffer. Bound proteins were then eluted using 0.1 M glycine, subjected to mass spectrometry analysis.

ORGANISM(S): Homo Sapiens

SUBMITTER: Shunji Jia  

PROVIDER: PXD078211 | iProX | Sat May 09 00:00:00 BST 2026

REPOSITORIES: iProX

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