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Proximity labeling of autophagic proteins to investigate the Arabidopsis thaliana autophagy regulatory network

ABSTRACT: In order to explore the regulatory network of plant autophagy, this study generated stable transgenic lines expressing ATG5-TurboID, ATG7-TurboID and ATG11-TurboID. Autophagy was induced by combined treatment with the autophagy inducer AZD8055 and biotin. Proximal proteins of autophagy-related proteins under autophagic conditions were enriched using immunoprecipitation coupled with mass spectrometry (IP-MS), followed by network analysis to identify potential interacting proteins and functional modules. This study provides a richer range of candidate targets for further dissecting the autophagy regulatory network in Arabidopsis thaliana.

ORGANISM(S): Arabidopsis Thaliana

SUBMITTER: Lei Li

PROVIDER: PXD078545 | iProX | Tue May 19 00:00:00 BST 2026

REPOSITORIES: iProX

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