Proteomics

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Chemoproteomic profiling of SGPP-binding proteins


ABSTRACT: S-guanylation is an endogenous post translational modification that has been implicated as a specific autophagic signal, yet its molecular mechanism and the identity of its binding proteins remain largely unknown. Here, we developed the first diazirine based photoaffinity probe for S-guanylation (SGPP) to capture and identify its interacting proteins in living cells. The SGPP efficiently labeled target proteins in a time , concentration , and UV crosslinking dependent manner, as demonstrated by in gel fluorescence scanning. Competitive labeling with excess FBnG, a known AUTAC warhead, confirmed the specificity of the probe. Using a chemoproteomic workflow combining SGPP mediated photocrosslinking, affinity enrichment, and mass spectrometry, we identified candidate binding proteins in HeLa cells

ORGANISM(S): Homo Sapiens

SUBMITTER: Han Wei  

PROVIDER: PXD078661 | iProX | Thu May 21 00:00:00 BST 2026

REPOSITORIES: iProX

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