Proteomics

Dataset Information

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Identification of Nanchangmycin protein targets

ABSTRACT: HeLa cells were treated with Nan-Dyne, a nanchangmycin probe bearing both alkyne and diazirine, irradiated with UV. For competition experiments, cells were also treated with an excess of nanchangmycin. Cells were irradiated with UV, lysed, clicked with azido-biotin, and proteins enriched on neutravidin beads. Samples were eluted on a SDS-Page gel and in-gel digested with trypsin.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Cervix Carcinoma

SUBMITTER: EMMANUELLE THINON  

LAB HEAD: Emmanuelle Thinon

PROVIDER: PXD062176 | Pride | 2025-11-10

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
combined_protein.tsv Tabular
et220301b_07.pepXML Pepxml
et220301b_07.raw Raw
et220301b_08.pepXML Pepxml
et220301b_08.raw Raw
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Publications

Affinity-based protein profiling of the antiviral natural product nanchangmycin.

Leiva Santiago S   Freyermuth Chloé C   Claverol Stéphane S   Mantione Daniele D   Thinon Emmanuelle E  

RSC chemical biology 20251021

Nanchangmycin is a natural product with broad-spectrum activity against various organisms, exhibiting antibiotic, antiviral, anticancer, and antifibrotic effects. Nanchangmycin belongs to the family of polyether ionophores and is proposed to exert its therapeutic effects by altering ion gradients across biological membranes. Although this therapeutic mechanism has been well characterised in cancer models, it does not fully explain how nanchangmycin inhibits Zika virus infection, as recently repo  ...[more]

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