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Mannose-Modified Lipid Nanoparticles Loaded with siZDHHC9 Remodel the Ras-ERK Signaling Axis in Antigen-Presenting Cells to Correct Th17/Treg Imbalance and Alleviate Fusobacterium nucleatum-Driven Ulcerative Colitis

ABSTRACT: Fusobacterium nucleatum-induced ulcerative colitis (UC) is characterized by persistent intestinal inflammation and an imbalance in immune homeostasis, in which abnormal activation of antigen-presenting cells (APCs) and a Th17/Treg imbalance play critical roles in disease progression. This study aimed to construct the mannose-modified lipid nanoparticles (LNPs) loaded with siZDHHC9 (Man-LNP@siZDHHC9). By integrating single-cell RNA sequencing (scRNA-seq) and multi-omics technologies, we systematically elucidated the molecular mechanisms by which Man-LNP@siZDHHC9 remodels APC-T cell interactions by inhibiting the HRas palmitoylation-mediated ERK signaling axis, thereby ameliorating Th17/Treg imbalance and alleviating Fusobacterium nucleatum-induced UC.

ORGANISM(S): Mus Musculus

SUBMITTER: Shanling Xu

PROVIDER: PXD081963 | iProX | Fri Jul 31 00:00:00 GMT+01:00 2026

REPOSITORIES: iProX

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