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DiPIUS_Fbxw7

ABSTRACT: Although elucidation of enzyme–substrate relations is fundamental to the advancement of biology, universal approaches to the identification of substrates for a given enzyme have not been established. It is especially difficult to identify substrates for ubiquitin ligases, given that most such substrates are immediately ubiquitylated and degraded as a result of their association with the enzyme. We here describe the development of a new approach, DiPIUS (differential proteomics-based identification of ubiquitylation substrates), to the discovery of substrates for ubiquitin ligases. We applied DiPIUS to Fbxw7α, and identified candidate substrates including previously known targets. DiPIUS is thus a powerful tool for unbiased and comprehensive screening for substrates of ubiquitin ligases.

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Keiichi I. Nakayama

PROVIDER: PXD008705 | JPOST Repository | Tue Mar 06 00:00:00 GMT 2018

REPOSITORIES: jPOST

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			Action	DRS
	T110419_A01-KT.RAW	Raw
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	T110419_A03-KT.RAW	Raw
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