Simulated spectral library searching on TripleTOF phosphopeptide datasets

ABSTRACT: Synthetic phosphopeptides and enriched K562 cell phosphopeptides were analyzed using a TripleTOF 6600 mass spectrometer before and after enzymatic dephosphorylation. Dephosphorylated peptides identified by X!Tandem database searching were subjected to spectral simulation of all possible single phosphorylations using SimPhospho software. Phosphopeptides were identified and localized by SpectraST searching against a library of the simulated spectra, and also by MaxQuant database searching.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Susumu Y. Imanishi 

PROVIDER: PXD012433 | JPOST Repository | Tue Dec 03 00:00:00 GMT 2019

REPOSITORIES: jPOST