Ontology highlight
ABSTRACT:
ORGANISM(S): Homo Sapiens (human)
SUBMITTER: Mio Iwasaki
PROVIDER: PXD014527 | JPOST Repository | Thu Jan 30 00:00:00 GMT 2020
REPOSITORIES: jPOST
Gee Peter P Lung Mandy S Y MSY Okuzaki Yuya Y Sasakawa Noriko N Iguchi Takahiro T Makita Yukimasa Y Hozumi Hiroyuki H Miura Yasutomo Y Yang Lucy F LF Iwasaki Mio M Wang Xiou H XH Waller Matthew A MA Shirai Nanako N Abe Yasuko O YO Fujita Yoko Y Watanabe Kei K Kagita Akihiro A Iwabuchi Kumiko A KA Yasuda Masahiko M Xu Huaigeng H Noda Takeshi T Komano Jun J Sakurai Hidetoshi H Inukai Naoto N Hotta Akitsu A
Nature communications 20200313 1
Prolonged expression of the CRISPR-Cas9 nuclease and gRNA from viral vectors may cause off-target mutagenesis and immunogenicity. Thus, a transient delivery system is needed for therapeutic genome editing applications. Here, we develop an extracellular nanovesicle-based ribonucleoprotein delivery system named NanoMEDIC by utilizing two distinct homing mechanisms. Chemical induced dimerization recruits Cas9 protein into extracellular nanovesicles, and then a viral RNA packaging signal and two sel ...[more]