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Crosslinking/mass spectrometry (CLMS) analysis of Tetrahymena ODA-Q22MS1 complex

ABSTRACT: The outer dynein arms (ODAs) are the main force generators in cilia. Crosslinking/mass spectrometry (CLMS) was applied to investigate the interaction between ODA and its binding factor Q22MS1.

ORGANISM(S): Tetrahymena

SUBMITTER: Juri Rappsilber

PROVIDER: PXD022936 | JPOST Repository | Tue Dec 07 00:00:00 GMT 2021

REPOSITORIES: jPOST

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			Action	DRS
	B201121_02_Lumos_ZC_CO_140_ODA-MS1_BS3_SECf5.raw	Raw
	B201121_03_Lumos_ZC_CO_140_ODA-MS1_BS3_SECf6.raw	Raw
	B201121_04_Lumos_ZC_CO_140_ODA-MS1_BS3_SECf7-1.raw	Raw
	B201121_05_Lumos_ZC_CO_140_ODA-MS1_BS3_SECf7-2.raw	Raw
	B201121_06_Lumos_ZC_CO_140_ODA-MS1_BS3_SECf8.raw	Raw

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Publications

Shulin packages axonemal outer dynein arms for ciliary targeting.

Mali Girish R GR Ali Ferdos Abid FA Lau Clinton K CK Begum Farida F Boulanger Jérôme J Howe Jonathan D JD Chen Zhuo A ZA Rappsilber Juri J Skehel Mark M Carter Andrew P AP

Science (New York, N.Y.) 20210201 6532

The main force generators in eukaryotic cilia and flagella are axonemal outer dynein arms (ODAs). During ciliogenesis, these ~1.8-megadalton complexes are assembled in the cytoplasm and targeted to cilia by an unknown mechanism. Here, we used the ciliate <i>Tetrahymena</i> to identify two factors (Q22YU3 and Q22MS1) that bind ODAs in the cytoplasm and are required for ODA delivery to cilia. Q22YU3, which we named Shulin, locked the ODA motor domains into a closed conformation and inhibited motor ...[more]

PMID: 33632841

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