Dataset Information

Co-immunoprecipitated human proteins with tobacco NtFT4

ABSTRACT: Biopharmaceutical proteins are produced in mammalian cells by transient expression or stable transformation, but both processes require robust and viable cells. Cell line engineering must therefore balance improved cell growth and viability with high productivity. We tested the expression of non-mammalian phosphatidylethanolamine binding proteins to enhance cell proliferation. The tobacco protein NtFT4 improved the proliferation and viability of HEK-293T cells in animal component-free suspension cultures. Viable cell density is usually impaired by efficient transfection, but we found that the number of HEK-293TNtFT4 cells doubled at the peak of protein expression and the antibody yield increased by approximately one third. The improved growth and viability were observed in different cell backgrounds, suggesting the system is transferable. Furthermore, high densities of viable HEK-293TNtFT4 cells will allow further modifications to boost protein synthesis. The established HEK-293TNtFT4 cell line provides a new human cell platform that increases cell proliferation without sacrificing translational activity.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Philip Kaenel

PROVIDER: PXD023020 | JPOST Repository | Fri Dec 10 00:00:00 GMT 2021

REPOSITORIES: jPOST

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			Action	DRS
	190131_C19_008_QHF1_PTM_Rev01_%2319_006_PAP254.raw	Raw
	190131_C19_008_QHF1_PTM_Rev01_%2319_006_PAP255.raw	Raw
	190131_C19_008_QHF1_PTM_Rev01_%2319_006_PAP256.raw	Raw
	190131_C19_008_QHF1_PTM_Rev01_%2319_006_PAP257.raw	Raw
	190131_C19_008_QHF1_PTM_Rev01_%2319_006_PAP258.raw	Raw

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Publications

The tobacco phosphatidylethanolamine-binding protein NtFT4 increases the lifespan of <i>Drosophila melanogaster</i> by interacting with the proteostasis network.

Känel Philip P Noll Gundula A GA Schroedter Katrin K Naffin Elke E Kronenberg Julia J Busswinkel Franziska F Twyman Richard M RM Klämbt Christian C Prüfer Dirk D

Aging 20220408 7

Proteostasis reflects the well-balanced synthesis, trafficking and degradation of cellular proteins. This is a fundamental aspect of the dynamic cellular proteome, which integrates multiple signaling pathways, but it becomes increasingly error-prone during aging. Phosphatidylethanolamine-binding proteins (PEBPs) are highly conserved regulators of signaling networks and could therefore affect aging-related processes. To test this hypothesis, we expressed PEPBs in a heterologous context to determi ...[more]

PMID: 35396341

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