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Data independent acquisition mass spectrometry of isolated glomeruli from mutant mice lacking the nuclear export signal of p62 and WT

ABSTRACT: ＜Project description＞ Data independent acquisition (DIA) mass spectrometry of Isolated glomeruli from homozygous deletion of the nuclear export signal of p62 (dNES) and WT mice (n=3 each). ＜Sample processing＞ Formalin fixed paraffin embedded (FFPE) tissues were prepared in general method and kidney samples were cut into 5µm-thick sections on the membrane slides (Leica Microsystems, Mannheim, Germany). Glomeruli were isolated via laser capture micro-dissection (0.6 mm2 in total). The dissected glomeruli were collected separately into 40 µL of Tris EDTA buffer and 0.002% Zwittergent 3-16 detergent.　Solubilised proteins were further digested into peptides by 0.01ug/ul trypsin (Promega, Madison, WI, USA) overnight. Each sample was centrifuged at 15000 rpm for 30 minutes prior to measurement. ＜Mass spectrometry and data processing＞ Samples were measured by DIA-MSMS using NanoLC-MSMS system with Vanquish Neo and Orbitrap Exploris 240 (Thermo Scientific). The measurement data were analyzed with DIA-NN 1.8.1.

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Eiji Warabi

PROVIDER: PXD039935 | JPOST Repository | Thu Feb 08 00:00:00 GMT 2024

REPOSITORIES: jPOST

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