Project description:This dataset contains label-free quantitative LC-MS/MS data from a comparative DNA affinity pull-down experiment. Two different 5′-biotinylated DNA probes were incubated with nuclear protein extracts from MCF-7 cells. Probe-associated protein complexes were enriched with streptavidin magnetic beads, eluted, digested and analyzed by nanoflow LC-MS/MS. Protein abundance in the target-probe group was compared with that in the reference-probe group to identify proteins showing preferential association with the selected DNA sequence. The submitted files include raw mass spectrometry data and processed protein identification and quantification results.
Project description:Diaminoquinazolines represent a privileged scaffold for antimalarial discovery, including use as putative Plasmodium histone lysine methyltransferase inhibitors (BIX-01294). Despite this, robust evidence for their molecular targets, proteome-wide, is lacking. Here we report the design and development of a small-molecule photo-crosslinkable probe to investigate the targets of our diaminoquinazoline series. We demonstrate the effectiveness of our designed probe for photoaffinity labelling of Plasmodium lysates and initial pull-down proteomics experiments identified proteins from different classes enriched by the probe, highlighting the suitability of the developed probe as a valuable tool for protein identification in Plasmodium falciparum.
Project description:Given the pivotal role of Tat in HIV-1 latency, we performed RNA pull-down assays in latently HIV-1-infected J-Lat 10.6 cells using biotinylated RNA probes (including a control RNA probe and a Tat-specific RNA probe) . Cell lysates were incubated with streptavidin agarose resin pre-bound to Tat RNA probes, and the enriched Tat RNA-binding proteins were separated by SDS-PAGE and analyzed by liquid chromatography mass spectrometry (LC-MS/MS)