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Identification of FBP1 binding proteins in wild-type, G164D and F194S comparisons by LC-MS/MS analysis

ABSTRACT: Myc-tagged FBP1-WT, Myc-tagged FBP1-G164D, Myc-tagged FBP1-F194S or empty vector were transfected into FBP1-KO HepG2 cells. FBP1 complexes were immunoprecipitated with anti-Myc beads and then separated by SDS‒PAGE. Bands were removed from the gel, and trypsin-digested samples were subjected to liquid chromatography-tandem mass spectrometry (LC-MS/MS) using an Orbitrap Velos Pro (Thermo Scientific). MS/MS dataset analysis was performed using the Mascot software program (Matrix Science).

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Tomoaki Tanaka

PROVIDER: PXD042364 | JPOST Repository | Sat May 20 00:00:00 BST 2023

REPOSITORIES: jPOST

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