Project description:JMJD2A was chromatin immunoprecipitated from HEK293T cells overexpressing GFP-JMJD2A. From Van Rechem et al. 2011 JBC Determination of JMJD2A binding sites by MA2C analysis
Project description:Ubiquitin Specific Peptidase 7 (USP7) is a deubiquitinase of several important regulatory proteins, and important regulator of TGFb pathway. To investigate their role in cell signaling, we analyzed global mRNA levels in HEK293T cells that were knocked down with shRNAs against USP7 and non-targeting control (CTRL).
Project description:Glycoproteins isolated from elongating cotton fiber cells were analyzed using 2D-PAGE followed by MALDI TOF/TOF based protein identification. Protein isoforms were identified using Tandem mass spectrometry. Lectin based Glycopeptide enrichment strategy followed by PNGase catalyzed deglycosylation reaction was employed to assign potential N-linked glycosylation sites.
Project description:To identify ZNF506 genome-wide target sites, we performed ChIP-seq assay and found that ZNF506 binding sites enriched at PBS-Pro-containing ERV subfamilies (ERVPs) and further motif calling analysis showed that ZNF506 binds to PBS-Pro sequences, promoting formation of H3K9me3 modifications at binding regions. And ChIP-seq assay also indicated that the distinction of H3K9me3 signals closed to ZNF506 peak regions between ZNF506 overexpressing (OE) HEK293T cells and ZNF506 Knockout (KO) HEK293T cells was correlated with the different recruitment of corepressor KAP1. The ChIP-seq experiments using GFP antibodies and H3K9me3 antibodies on ZNF506-GFP OE HEK293T cell lines, and ZNF417-GFP OE HEK293T cell lines were used as controls for H3K9me3 ChIP. Also, the ChIP-seq experiments were performed using H3K9me3 antibodies and KAP1 antibodies on ZNF506 KO HEK293T cells and ZNF506 OE HEK293T cells.
Project description:Microarray-based gene expression analysis identified genes differentially expressed in HEK293T cells after CHMP5 gene modulation. In this study, HEK293T cells, silenced for CHMP5 using sh-CHMP5 was compared to genome wide expression changes when compared to cellular transfections with a sh-luciferase control by Significance Analysis of Microarray data. Further, overexpression of CHMP5 using the vector pCMV_CHMP5 was compared to cellular transfections with an empty vector pCMV in the cell line HEK293T
Project description:Purpose: Comparison of RNA-sequencing datasets obtained from exosomes of Nef-transfected and Mock-transfected HEK293T cells Methods: Assessment of RNA content of exosomes produced by Nef-transfected HEK293T cells and and Mock-transfected HEK293T cells Results: Differences in a set of microRNAs Conclusions: Nef-transfection induces changes in the microRNA content of exosomes