Project description:This study presents an alternative preliminary strategy to identify and quantify serum hFSH glycoforms based on immunopurification assay and mass spectrometry (MS) using parallel reaction monitoring (PRM) analysis. In this study, we provide MS-PRM data acquisition method for hFSH glycopeptides identification with high specificity and their quantification by extracting the chromatographic traces of selected fragments of glycopeptides. Once set up in all its features, the proposed method could be transferred to the clinic to improve the fertility treatments and follow-ups in men and women.
Project description:A comprehensive screening of glycopeptides as candidate biomarkers from human serum for early diagnosis of NASH hepatocellular carcinoma using a stepped HCD method and PRM evaluation. Glycopeptides from vitronectin(VTNC) has been reported as biomarkers for NASH-related HCCs.
Project description:Combining in-silico analysis, serum high abundant protein depletion, and DDA and PRM acquisitions, we established a simple workflow to select PRM high response peptides for protein quantification in serum. The workflow enables evaluating peptide intensity, MS2 match with the spectral library, peptide stability, and linearity of response to different injected masses. This workflow was successfully applied to twelve human complement system proteins. Besides, we employed synthetic labelled peptides to compensate inter LC-MS run variability, improving the quantification. We could demonstrate this method is useful and applicable for quantification of diverse targets with time optimization and accuracy insurance by quantitative proteomics technology.
Project description:The ProteomeTools project aims to derive molecular and digital tools from the human proteome to facilitate biomedical and life science research. Here, we describe the the generation and multimodal LC-MS/MS analysis of >500,000 tryptic synthetic peptides labeled with tandem mass tags (TMT6plex).