Proteomics

Dataset Information

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CRISPR-assisted RNA-protein interaction detection (CARPID) combined with quantitative mass spectrometry was used to identify proteins interacting with the long non-coding RNA (lncRNA) Taurine Up-regulated 1 (TUG1) in HEK293T cells under physiological conditions and after Camptothecin (CPT) treatment.


ABSTRACT: This project utilized CRISPR-assisted RNA-protein interaction detection (CARPID) combined with mass spectrometry to identify proteins interacting with the long non-coding RNA (lncRNA) TUG1 in HEK293T cells. TUG1 preferentially interacts with known R-loop-associated proteins, particularly under Camptothecin (CPT) treatment. Additionally, we identified interacting proteins for the lncRNAs MALAT1 to validate the binding specificity of TUG1.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Yutaka Kondo 

PROVIDER: PXD049137 | JPOST Repository | Thu Jul 17 00:00:00 BST 2025

REPOSITORIES: jPOST

Dataset's files

Source:
Action DRS
SN220410_12ul.raw Raw
SN220411_12ul.raw Raw
SN220412_12ul.raw Raw
SN220413_12ul.raw Raw
SN220414_12ul.raw Raw
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