Proteomics

Dataset Information

0

Cold Atmospheric Plasma Jet Activated Medium Promotes Wound Healing through CK2-mediated ERK and AKT signaling pathways


ABSTRACT: In this study, we aimed to explore the molecular mechanisms of wound healing affected by CAPJ using the parallel MS-based proteome and phosphoproteome. The keratinocyte, HaCaT, was used as cell mode to treat CAPJ indirectly by employing plasma activated medium (PAM) which was culture medium explored to He/Ar-generated CAPJ following previous study’s procedures. The datasets were integrated and systematically analyzed to dissect molecular and signaling pathway alternation in keratinocytes during PAM intervention. The results showed that PAM treatment significantly activated ERK, CK2 and AKT kinases and the phosphorylation-dependent signaling pathways of PI3K/AKT/mTOR and MEK/ERK were multiaxially disturbed, in which CK2 was suggested to play a coordination role between these two pathways. Collectively, this knowledge allows for a better understanding of how CAPJ regulates keratinocyte signaling during wound closure, and provides new insights into the development of clinical skin wound treatments.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Miao-Hsia Lin 

PROVIDER: PXD050065 | JPOST Repository | Sat Feb 22 00:00:00 GMT 2025

REPOSITORIES: jPOST

Dataset's files

Source:
Action DRS
20201201_Ctrl1_phospho_01.raw Raw
20201201_Ctrl1_phospho_02.raw Raw
20201201_Ctrl1_prot_01.raw Raw
20201201_Ctrl1_prot_02.raw Raw
20201201_Ctrl2_phospho_01.raw Raw
Items per page:
1 - 5 of 20
altmetric image

Publications

Cold Atmospheric Plasma Jet Promotes Wound Healing Through CK2-Coordinated PI3K/AKT and MAPK Signaling Pathways.

Wu Pei-Shan PS   Wong Tzu-Hsuan TH   Hou Chun-Wei CW   Chu Teng-Ping TP   Lee Jyh-Wei JW   Lou Bih-Show BS   Lin Miao-Hsia MH  

Molecular & cellular proteomics : MCP 20250403 5


The promising role of cold atmospheric plasma jet (CAPJ) treatment in promoting wound healing has been widely documented in therapeutic implications. However, the fact that not all subjects respond equally to CAPJ necessitates the investigation of the underlying cellular mechanisms, which have been rarely understood so far. Given that wound healing is a complex and prolonged process, post plasma-activated medium (PAM) treated keratinocytes were collected at two time points, 2 h (receiving) and 2  ...[more]

Similar Datasets

2021-12-22 | MSV000088606 | GNPS
2024-09-02 | BIOMD0000000653 | BioModels
2024-09-02 | BIOMD0000000656 | BioModels
2024-09-02 | BIOMD0000000654 | BioModels
2024-09-02 | BIOMD0000000655 | BioModels
2024-09-02 | BIOMD0000000652 | BioModels
2024-09-02 | BIOMD0000000648 | BioModels
2025-04-17 | PXD051544 | JPOST Repository
2020-02-28 | GSE132945 | GEO
2017-03-31 | MSV000080844 | MassIVE