Project description:In order to investigate the function and mechanism of HemSC-EVs, we performed RNA-seq of HemSC-EVs which were extracted from cell culture supernatant of HemSC.
Project description:EVs in culture supernatant can be concentrated with removing exsomeres and 97% free proteins by MWCO 750 kDa UF. DEAE chromatography can be divided into bioactive EXO and other EVs as nucleic acid (DNA) cargo in UF-concentrated EVs.
Project description:EVs in culture supernatant can be concentrated with removing exsomeres and 97% free proteins by MWCO 750 kDa UF. DEAE chromatography can be divided into bioactive EXO and other EVs as nucleic acid (DNA) cargo in UF-concentrated EVs.
Project description:EVs in culture supernatant can be concentrated with removing exsomeres and 97% free proteins by MWCO 750 kDa UF. DEAE chromatography can be divided into bioactive EXO and other EVs as nucleic acid (DNA) cargo in UF-concentrated EVs.
Project description:BMECs-derived exosomes were separated from the culture supernatant of BMECs by a combined ultracentrifugation approach, and the purity of exosomes was identified by Western blotting analysis. LC-MS/MS identified 638 identified proteins in BMECs-derived exosomes.
2020-05-12 | PXD019130 |
Project description:miRNA analysis of EVs from Babesia divergens culture supernatant
Project description:Exosomes transferred from the T cell to the APC contain many different types of biologically active molecules, including proteins and genetic material. To investigate the possible specific function of the different components of the biologic material transferred from the T cell to the DC, we have characterized the protein and genetic content of EVs isolated from the culture supernatant of primary T lymphoblasts by differential ultracentrifugation
Project description:Samples of EE-EVs and RE-EVs, isolated by ultracentrifugation, were analyzed using data-independent acquisition mass spectrometry (DIA-MS).
Project description:Under physiological conditions, extracellular vesicles (EVs) are present simultaneously in the extracellular compartment together with cytokines. Thus, we hypothesized that EVs in combination with cytokines induce different responses of monocyte cells compared to EVs or cytokines alone. Human monocyte U937 cells were incubated with EV-containing or EV-free CCRF human T-cell supernatant, with or without the addition of TNF. U937 cells cultured in EV-free supernatant, supernatant containing CCRF t-cell derived EVs, TNF or both. Each treatment option was measured in 3 replicates.