Dataset Information

Mass spectrometry analysis of venom gland components of the parasitoid wasp Asobara japonica

ABSTRACT: (1) Secreted materials from the venom gland: 50 venom glands were cultured in 20 μL PBS for 2 hours. The cultured media were collected. Three biological replications were analyzed. (2) Lysates of the venom glands: 50 venom glands were squashed in 50 μL PBS and centrifuged. The supernatants were collected. Three biological replications were analyzed.

ORGANISM(S): Cellular Organisms

SUBMITTER: Ryusuke Niwa

PROVIDER: PXD052039 | JPOST Repository | Wed May 07 00:00:00 BST 2025

REPOSITORIES: jPOST

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Publications

Parasitoid wasp venoms degrade Drosophila imaginal discs for successful parasitism.

Kamiyama Takumi T Shimada-Niwa Yuko Y Mori Hitoha H Tani Naoki N Takemata-Kawabata Hitomi H Fujii Mitsuki M Takasu Akira A Katayama Minami M Kuwabara Takayoshi T Seike Kazuki K Matsuda-Imai Noriko N Senda Toshiya T Katsuma Susumu S Nakamura Akira A Niwa Ryusuke R

Science advances 20250129 5

Parasitoid wasps, one of the most diverse and species-rich animal groups on Earth, produce venoms that manipulate host development and physiology to exploit resources. However, mechanisms of actions of these venoms remain poorly understood. Here, we discovered that the endoparasitoid wasp, Asobara japonica, induces apoptosis, autophagy, and mitotic arrest in the adult tissue precursors of its host Drosophila larvae. We termed this phenomenon imaginal disc degradation (IDD). A multi ...[more]

PMID: 39879297

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Project description:Background The generalist dipteran pupal parasitoid Nasonia vitripennis injects 79 venom peptides into the host before egg laying. This venom induces several important changes in the host, including developmental arrest, immunosuppression, and alterations to normal metabolism. It is hoped that diverse and potent bioactivities of N. vitripennis venom provide an opportunity for the design of novel acting drugs. However, currently very little is known about the individual functions of N. vitripennis venom peptides and less than half can be bioinformatically annotated. The paucity of annotation information complicates the design of studies that seek to better understand the potential mechanisms underlying the envenomation response. Although the RNA interference system of N. vitripennis provides an opportunity to functionally characterise venom encoding genes, with 79 candidates this represents a daunting task. For this reason we were interested in determining the expression levels of venom encoding genes in the venom gland, such that this information could be used to rank candidate venoms. To do this we carried out deep sequencing of the transcriptome of the venom gland and neighbouring ovary tissue and used RNA-seq to measure expression from the 79 venom encoding genes. The generation of a specific venom gland transcriptome dataset also provides further opportunities to investigate novel features of this highly specialised organ. Results High throughput sequencing and RNA-seq revealed that the highest expressed venom encoding gene in the venom gland was a serine protease called Nasvi2EG007167, which has previously been implicated in the apoptotic activity of N. vitripennis venom. As expected the RNA-seq confirmed that the N. vitripennis venom encoding genes are almost exclusively expressed in the venom gland relative to the neighbouring ovary tissue. Novel peptides appear to perform key roles in N. vitripennis venom function as only four of the highest 15 expressed venom encoding genes are bioinformatically annotationed. The high throughput sequencing data also provided evidence for the existence of an additional 471 novel genes in the Nasonia genome that are expressed in the venom gland and ovary. Finally, metagenomic analysis of venom gland transcripts identified viral transcripts that may play an important part in the N. vitripennis venom function. Conclusions The expression level information provided here for the 79 venom encoding genes provides an unbiased dataset that can be used by the N. vitripennis community to identify high value candidates for further functional characterisation. These candidates represent bioactive peptides that have value in drug development pipelines.

			Action	DRS
	NR7-8_Asobara_japonica.xlsx	Xlsx
	NR7_L02.raw	Raw
	NR7_L03.raw	Raw
	NR7_L05.raw	Raw
	NR7_L06.raw	Raw

Dataset Information

Mass spectrometry analysis of venom gland components of the parasitoid wasp Asobara japonica

Dataset's files

Publications

Parasitoid wasp venoms degrade <i>Drosophila</i> imaginal discs for successful parasitism.

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Dataset Information

Mass spectrometry analysis of venom gland components of the parasitoid wasp Asobara japonica

Dataset's files

Publications

Parasitoid wasp venoms degrade &lt;i&gt;Drosophila&lt;/i&gt; imaginal discs for successful parasitism.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Parasitoid wasp venoms degrade <i>Drosophila</i> imaginal discs for successful parasitism.