Venom proteome data of two egg parasitoids

ABSTRACT: After the venom gland synthesizes, venom proteins will be stored in its venom sacs. So, we dissected venom sacs to obtain the venom. Three replicates of 400 females of both parasitoid species were taken respectively. The venom sacs were dissected and separated from the end of the abdomen and washed three times in sterile 1× Pringle's phos-phate-buffered saline buffer (1×PBS) (Biosharp, China). The venom sacs were punctured with the tip of dissecting forceps and dissolved in 1×PBS buffer and protease inhibitor (TransGen Biotech), centrifuged at 12,000g for 15 min at four °C. The supernatant was collected, frozen in liquid nitrogen, and stored at -80 °C for proteome sequencing. The project uses Label free quantitative proteomics technology to conduct research. A total of 850 proteins were identified in three samples, including 782 quantitative proteins. Based on the above data, a systematic bioinformatics analysis was conducted on all identified proteins (protein function annotation)

ORGANISM(S): Anastatus Japonicus Mesocomys

SUBMITTER: Liansheng Zang  

PROVIDER: PXD041117 | iProX | Sat Mar 25 00:00:00 GMT 2023

REPOSITORIES: iProX