Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Label-free proteomics of cardiomyocytes USP5 and BioID

ABSTRACT: Label-free proteomics of cardiomyocytes WT and USP5 KO, BioID

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

SUBMITTER: Thomas Braun

PROVIDER: PXD057062 | JPOST Repository | Wed Oct 23 00:00:00 BST 2024

REPOSITORIES: jPOST

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	20160309_SJ_Mareike_CM_USP5_Ctrl1_45.raw	Raw
	20160309_SJ_Mareike_CM_USP5_Ctrl1_46.raw	Raw
	20160309_SJ_Mareike_CM_USP5_Ctrl1_47.raw	Raw
	20160309_SJ_Mareike_CM_USP5_Ctrl1_48.raw	Raw
	20160309_SJ_Mareike_CM_USP5_Ctrl1_49.raw	Raw

Items per page:

1 - 5 of 20

Similar Datasets

Label-free single-cell RNA Multiplexing leveraging genetic variability

Project description:Label-free single-cell RNA Multiplexing leveraging genetic variability

| PRJNA1040187 | ENA

BioID-Esrrg Overexpresssion in SH-SY5Ys [H202SC21031899]

Project description:SH-SY5Y cells were transfected with either BioID-GFP-NLS control or BioID-Flag-ERRg expressing adenoviruses and isolated with Trizol after 48 hours to compare gene expression and find potential ERRg targets specific to neuron-like cells.

2025-03-01 | GSE198215 | GEO

Label-free melanoma phenotype classification using AI-based morphological profiling

Project description:Label-free melanoma phenotype classification using AI-based morphological profiling

| PRJNA1140860 | ENA

Label-free quantitative proteomics analysis of bone marrow cells of MK-LCM WT versus KO mice

Project description:High ploidy large cytoplasmic megakaryocytes (LCM) are critical negative regulators of hematopoietic stem cells (HSC) and are responsible for platelet formation. Using a mouse knockout model with normal megakaryocyte numbers but essentially devoid of LCM (MK-LCM KO), we demonstrated a pronounced increase in bone marrow HSC concurrent with endogenous mobilization and extramedullary hematopoiesis. When HSC isolated from a MK-LCM KO microenvironment were transplanted in lethally irradiated mice, the absence of LCM increased HSC in BM, blood and spleen. Severe thrombocytopenia was observed in animals with diminished LCM, although there was no change in megakaryocyte ploidy distribution. In contrast, WT HSC-generated LCM regulated a normal HSC pool and prevented thrombocytopenia. The present label-free quantitative LC-MSMS data was used to determine proteins that are differentially expressed in bone marrow cells of MK-LCM WT versus MK-LCM KO mice.

2023-05-10 | PXD040423 | Pride

Label-free single-cell RNA Multiplexing leveraging genetic variability [scRNA-seq]

Project description:Label-free single-cell RNA Multiplexing leveraging genetic variability [scRNA-seq]

| PRJNA1040191 | ENA

BioID-Esrrg Overexpresssion in SH-SY5Ys [H202SC21031899]

Project description:BioID-Esrrg Overexpresssion in SH-SY5Ys [H202SC21031899]

| PRJNA814232 | ENA

Wu iPSC-CM MM11 label-free proteomics

Project description:Label-free proteomics from iPSC-CMs and cardiomyocytes.

2025-08-26 | MSV000098942 | MassIVE

Label free proteomics of isolated lysosomes from a wild type and LIMP2 knock out mouse

Project description:Quantitative label free proteomics on an n=1 experiment of isolated tritosomes (lysosomes) from mus musculus, WT and a knock out of the LIMP2 protein.

2018-11-27 | PXD001633 | Pride

Immune profiling and label-free detection of 2D MXenes at the single-cell and tissue level

Project description:There is a critical unmet need to image 2D materials within single cells and tissues. Here we propose a versatile label-free single-cell detection strategy based on mass cytometry. We use single-cell mass cytometry by time-of-flight (CyTOF), imaging mass cytometry, and multiplexed ion beam imaging by time-of-flight (MIBI-TOF) with three representative MXenes. Nb4C3, Mo2Ti2C3, and Ta4C3 were selected to ensure mass-detection within the range and to avoid overlap with currently available tags. We demonstrated their biocompatibility, detection, and quantification in 16 primary human immune cell subpopulations also showing the impact of MXene lateral size on the detection signal and cell binding. We report the biological and immune functional compatibility of MXenes. In vivo biodistribution experiments in mice using a mixture of MXenes revealed their presence in liver, blood, spleen, and lungs. Lastly, we applied MIBI-TOF to capture the presence of MXenes in different organs. The label-free detection of 2D materials by mass cytometry at the single cell level, on multiple subpopulations and in multiple organs in vivo opens exciting new opportunities in biomedicine.

2025-04-01 | GSE200036 | GEO

Label-free single-cell RNA Multiplexing leveraging genetic variability [bulk RNA-seq]

Project description:Label-free single-cell RNA Multiplexing leveraging genetic variability [bulk RNA-seq]

| PRJNA1040192 | ENA