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Tape-Stripped Proteomics: A Comparative Analysis of Different Sample Collection Approaches

ABSTRACT: The study explores the impact of sampling surrounding normal-looking skin and the amount of pressure applied during the collection of stratum corneum samples using adhesive discs, aiming to improve the reproducibility of proteomic data in skin research. Researchers collected samples from six participants, examining moles and nearby normal skin. Different pressures and marking techniques were used on three moles and normal skin, ensuring variation in sample collection methods. Proteins were then extracted, processed, and analyzed using mass spectrometry and specialized software to identify and quantify proteins. Results were analyzed for differential protein abundance, providing insights into the optimal collection conditions for more accurate skin protein profiling.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Ali Azimi

PROVIDER: PXD057504 | JPOST Repository | Wed Nov 05 00:00:00 GMT 2025

REPOSITORIES: jPOST

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			Action	DRS
	MEL01_L2.raw	Raw
	MEL01_L2S.raw	Raw
	MEL01_L3.raw	Raw
	MEL01_NS.raw	Raw
	MEL02_L1.raw	Raw

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