Dataset Information

Identification of phosphorylation sites and binding proteins of Saccharomyces cerevisiae Tda1

ABSTRACT: Tda1 in Saccharomyces cerevisiae is a protein kinase that is activated in response to glucose starvation. However, how it is activated has not been investigated for a long time. In this analysis, yeast cells were grown in high (2%) or low (0.05%) glucose medium, and the lysate were subjected to immunoprecipitation of Tda1-3xHA with an anti HA antibody. Proteins in the immnoprecipitated fractions were digested and peptides were analyzed by MS/MS.

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Takumi Kamura

PROVIDER: PXD060707 | JPOST Repository | Mon Mar 24 00:00:00 GMT 2025

REPOSITORIES: jPOST

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Dataset's files

Source:

			Action	DRS
	20230718_QE1_HTCPAL_sand_AcOH_PE_SUSValve_2023-092_e1.raw	Raw
	20230718_QE1_HTCPAL_sand_AcOH_PE_SUSValve_2023-092_e3.raw	Raw
	20240924_QE1_HTCPAL_sand_AcOH_PE_SUSValve_2023-092_e1_e3_withNorm.mzTab	Mztab
	20240924_QE1_HTCPAL_sand_AcOH_PE_SUSValve_2023-092_e1_e3_withNorm_ALL_PeptideGroups.xlsx	Xlsx
	20240924_QE1_HTCPAL_sand_AcOH_PE_SUSValve_2023-092_e1_e3_withNorm_ALL_Proteins.xlsx	Xlsx

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Publications

Snf1 and yeast GSK3-β activates Tda1 to suppress glucose starvation signaling.

Nonaka Kazuki K Nishimura Kohei K Uesaka Kazuma K Mishiro-Sato Emi E Fukase Minako M Kato Rei R Okumura Fumihiko F Nakatsukasa Kunio K Obara Keisuke K Kamura Takumi T

EMBO reports 20250424 11

In budding yeast, the presence of glucose, a preferred energy source, suppresses the expression of respiration-related genes through a process known as glucose repression. Conversely, under glucose starvation conditions, Snf1 phosphorylates and activates downstream factors, relieving this repression and allowing cells to adapt. Recently, the Tda1 protein kinase has been implicated in these glucose starvation responses, although its function remains largely uncharacterized. In this study, we demo ...[more]

PMID: 40275108

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Project description:Cultivation methods used to investigate microbial calorie restriction often result in carbon and energy starvation. This study aims to dissect cellular responses to calorie restriction and starvation in Saccharomyces cerevisiae by using retentostat cultivation. In retentostats, cells are continuously supplied with a small, constant carbon and energy supply, sufficient for maintenance of cellular viability and integrity but insufficient for growth. When glucose-limited retentostats cultivated under extreme calorie restriction were subjected to glucose starvation, calorie-restricted and glucose-starved cells were found to share characteristics such as increased heat-shock tolerance and expression of quiescence-related genes. However, they also displayed strikingly different features. While calorie-restricted yeast cultures remained metabolically active and viable for prolonged periods of time, glucose starvation resulted in rapid consumption of reserve carbohydrates, population heterogeneity due to appearance of senescent cells and, ultimately, loss of viability. Moreover, during starvation, calculated rates of ATP synthesis from storage carbohydrates were 2-3 orders of magnitude lower than steady-state ATP-turnover rates calculated under extreme calorie restriction in retentostats. Stringent reduction of ATP turnover during glucose starvation was accompanied by a strong down-regulation of genes involved in protein synthesis. These results demonstrate that extreme calorie restriction and carbon starvation represent different physiological states in S. cerevisiae. The yeast was first grown for 14 days under extreme calorie restriction in anaerobic, glucose-limited retentostats (Boender et al., 2009, Appl.Environ.Microbiol., 75: 5607-5614.). Subsequently, starvation was started by terminating the glucose feed. Yeast transcriptional reprogramming in response to calorie restriction and starvation was monitored by microarray analysis. Independent duplicate retentostat cultures, and subsequently starvation, were sampled for transcriptome analysis using Affymetrix microarrays. One time-point was sampled during calorie restriction (T0) and four time points were sampled during the starvation phase 10, 30, 60 and 120 minutes after switching of the feed, resulting in a dataset of 10 arrays.