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Ser2 phospholyration is suppressed by 7SK snRNA on snRNA for accurate termination. The mass data set of ICE1::AFF4 and ICE1 IP.

ABSTRACT: We prepared nuclear extracts from three types of HeLa cells: those expressing ICE1::AFF4, ICE1 alone, and untransduced control cells. Using V5-tag immunoprecipitation (IP), we aimed to compare the proteins associated with ICE1 and ICE1::AFF4. Our hypothesis is that the ICE1::AFF4 fusion protein will pull down more components of the Super Elongation Complex (SEC) than ICE1 alone, indicating enhanced interaction due to the presence of AFF4. This would suggest that AFF4 promotes SEC recruitment when fused to ICE1, potentially modulating transcriptional elongation.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Hidehisa Takahashi

PROVIDER: PXD062926 | JPOST Repository | Thu Jun 12 00:00:00 BST 2025

REPOSITORIES: jPOST

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			Action	DRS
	20240301Elite_HELA_ICE1.raw	Raw
	20240301Elite_HELA_ICE1_AFF4.raw	Raw
	20240301Elite_HELA_NC.raw	Raw
	ICE1_AFF4_HeLa.xlsx	Xlsx

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