ABSTRACT: HDAC8 was knocked down in HeLa cells. Peptides were dimethyl labeled and fractionated via SDB-RPS stage tips. After DIA, data was analysed via DIANN
Project description:APE1 was knocked down using siRNA in low passage patient-derived PDAC cells and the resulting cells, along with control cells were analysed using scRNA-seq to identify differentially expressed genes and pathways as a result of APE1 knock-down.
Project description:APE1 was knocked down using siRNA in low passage patient-derived PDAC cells and the resulting cells, along with control cells were analysed using scRNA-seq to identify differentially expressed genes and pathways as a result of APE1 knock-down.
Project description:ADGRG1/GPR56 was knocked down by two different shRNAs in cord blood CD34+ cells via lentiviral transduction. GFP was used as marker indicating successful transduction.
Project description:L1 elements are repeat elements known to get activated during hepatocarcinogenesis. We have knocked-down expression of L1 elements using shRNA targeting L1-ORF1 in Huh7 cell line and a control non-targeting cell line was also generated. Influence of L1 knockdown on overall cellular transcritome was then analysed by RNAseq followed by pathway analysis. Overall, a crosstalk between L1 elements and TGFbeta signaling pathway is observed.
Project description:Determination of accessible chromatin regions in the 8-cell stage mouse embryo after Suv39h2 knockdown The study aimed to address a potential function of H3K9me3 in early mouse development by assessing its impact on chromatin compaction. The histone methyltransferase responsible for de novo H3K9me3 at fertilization Suv39h2, was knocked down by microinjection of dsRNA targeting Suv39h2 in the early zygote. Embryos were then cultured until the 8-cell stage of development. They were then fixed and chromatin compaction was assessed by NicE-seq in pools of 10 8-cell stage embryos.
Project description:Yap1 and Wwtr1 were knocked down via siRNA in primary murine alveolar cells derived from normal or fibrotic mice (PBS treated as a control for bleomycin instilled animals, harvested at 14 days). Isolated cells from three independent isolations were plated on tissue culture plates and allowed to adhere for 2 days followed by incubation with siRNA via forward transfection. Samples were harvested 48 hours later for microarray analysis.
Project description:The abundance of miRNAs in control Xenopus tropicalis embryos at early gastrula stage 10.5 was compared to that in sibling embryos in which the RNA binding proteins lin28a1, lin28a2 and lin28b have been knocked down by injection of translation blocking antisense morpholino oligos.
Project description:To evaluate whether PLXDC2 mediated the role of PTEN on macrophages, PLXDC2 was knocked down in M2-polarized BMDMs, and the resultant PLXDC2-knocked down cells together with control cells were treated with or without PTEN, before subjected to global transcriptome profiling.