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Detecting protein higher-order structural changes using kinase as a phospho-labeler

ABSTRACT: We developed an in vitro kinase-based approach to detect proteome-wide protein conformational changes. By combining residue-specific phosphorylation with phosphopeptide enrichment, this method enables sensitive, site-specific structural profiling. We demonstrated that phosphorylation efficiency reflected structural differences and revealed conformational changes upon RNA digestion across the proteome.

ORGANISM(S): Homo Sapiens (human) Equus Caballus

SUBMITTER: Yasushi Ishihama

PROVIDER: PXD063369 | JPOST Repository | Mon Apr 27 00:00:00 BST 2026

REPOSITORIES: jPOST

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			Action	DRS
	211216AM02_aMb_RT_Src(-)_1.raw	Raw
	211216AM03_aMb_RT_Src(+)_1.raw	Raw
	211216AM16_aMb_RT_Src(-)_2.raw	Raw
	211216AM17_aMb_RT_Src(+)_2.raw	Raw
	211216AM30_aMb_RT_Src(-)_3.raw	Raw

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