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RIME of PbAP2-TR and PbMORC at the trophozoite stage

ABSTRACT: To explore the interaction-partners of PbAP2-TR and PbMORC in the asexual blood stage of Plasmodium berghei, rapid immunoprecipitation mass spectrometry of endogenous proteins (RIME) was performed, which is a combination of chromatin immunoprecipitation and liquid chromatography tandem-mass spectrometry. Parasites expressing GFP-fused PbAP2-TR and PbMORC were each generated from the Cas9-expressing parasite, and pbap2-g was then disrupted. Whole blood was harvested from mice infected with this transgenic parasite at 12 h post infection,and the parasites were then subjected to RIME experiments using anti-GFP antibody. Same procedures were performed for wild-type parasites as a control.

ORGANISM(S): Plasmodium Berghei

SUBMITTER: Tsubasa Nishi

PROVIDER: PXD067426 | JPOST Repository | Mon Dec 08 00:00:00 GMT 2025

REPOSITORIES: jPOST

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			Action	DRS
	F083189.mztab	Mztab
	F083397.mztab	Mztab
	F083398.mztab	Mztab
	F083399.mztab	Mztab
	F083400.mztab	Mztab

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