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Comparative proteomics analysis of chickpea and its wild relatives against Helicoverpa armigera infestation

ABSTRACT: Chickpea (Cicer arietinum L.) is an important legume crop, but its yield is greatly affected by the pod borer Helicoverpa armigera. Breeding for durable resistance has been limited by insufficient understanding of host–pest interactions. We conducted the first integrated proteomic analysis of chickpea and its pest, comparing resistant (ICCV506EB), susceptible (ICC3137), and wild relative (IG73016, C. cuneatum) genotypes. Resistant plants triggered rapid defense responses involving jasmonic acid signaling, transcriptional changes, and fatty acid–derived metabolites. In contrast, H. armigera activated detoxification and digestive modulation to counter defenses. Notably, larvae feeding on resistant wild relatives were impaired by linoleic acid derivatives acting as pro-toxins that disrupted growth and immunity. Our study uncovers the molecular basis of chickpea resistance and highlights wild relatives as a reservoir of defense traits. Enhancing early JA-pathway activation through breeding or biotechnology could help develop insect-resistant chickpea varieties, boosting productivity and sustainability.

ORGANISM(S): Cicer Arietinum

SUBMITTER: Dr Yogendra Kalenahalli

PROVIDER: PXD068327 | JPOST Repository | Sat Sep 13 00:00:00 BST 2025

REPOSITORIES: jPOST

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			Action	DRS
	Revanaya-CP-ICC3137-RM-1.mzML	Mzml
	Revanaya-CP-ICC3137-RM-2.mzML	Mzml
	Revanaya-CP-ICC3137-RM-3.mzML	Mzml
	Revanaya-CP-ICC3137-RP-1.mzML	Mzml
	Revanaya-CP-ICC3137-RP-2.mzML	Mzml

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Project description:Phytophthora cinnamomi Rands (Pc) is a hemibiotrophic oomycete and the causal agent of Phytophthora root rot (PRR) of the commercially important fruit crop avocado (Persea americana Mill.). Plant defense against pathogens is modulated by phytohormone signaling pathways such as salicylic acid (SA), jasmonic acid (JA), ethylene (ET), auxin and abscisic acid. The role of specific signaling pathways induced and regulated during hemibiotroph-plant interactions has been widely debated. Some studies report SA mediated defense while others hypothesize that JA responses restrict the spread of pathogens. This study aimed to identify the role of SA- and JA- associated genes in the defense strategy of a resistant avocado rootstock, Dusa® in response to Pc infection. Transcripts associated with SA-mediated defense pathways and lignin biosynthesis were upregulated at 6 hours post-inoculation (hpi). Results suggest that auxin, reactive oxygen species (ROS) and Ca2+ signaling was also important during this early time point, while JA signaling was absent. Both SA and JA defense responses were shown to play a role during defense at 18 hpi. Induction of genes associated with ROS detoxification and cell wall digestion (β-1-3-glucanase) was also observed. Most genes induced at 24 hpi were linked to JA responses. Other processes at play in avocado at 24 hpi include cell wall strengthening, the formation of phenolics and induction of arabinogalactan, a gene linked to Pc zoospore immobility. This study represents the first transcriptome wide analysis of a resistant avocado rootstock treated with SA and JA compared to Pc infection. The results provide evidence of a biphasic defense response against the hemibiotroph, which initially involves SA-mediated gene expression followed by the enrichment of JA-mediated defense from 18 to 24 hpi. Genes and molecular pathways linked to Pc resistance are highlighted and may serve as future targets for manipulation in the development of PRR resistant avocado rootstocks.

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Comparative proteomics analysis of chickpea and its wild relatives against Helicoverpa armigera infestation

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