Dataset Information

A biphasic molecular program orchestrates ontogenetic transitions and ecological specialization in invasive whitefly, Bemisia tabaci

ABSTRACT: The sweet potato whitefly, Bemisia tabaci, is a globally important pest that poses serious challenges to agriculture because of its remarkable ability to adapt and thrive in various environments. Despite its widespread presence, its development and growth remain poorly understood. In this study, we used a comprehensive multiomics approach, including small RNA (microRNA (miRNA)) sequencing, messenger RNA (mRNA) transcriptomics, and quantitative proteomics, to decipher the molecular developmental dynamics of B. tabaci at different life stages, such as eggs, nymphs, puparia (pupae), and adults. The findings revealed two principal ontogenetic stages in the life cycle of B. tabaci. The initial phase encompasses ontogenetic metamorphosis from the ovum to the inaugural nymphal instar, characterized by a decrease in matrilineal influence and an increase in metabolic activity. The subsequent phases occur as the organism advances its transition from the fourth-instar nymph stage to sexual maturity, resulting in profound metamorphic transformations and ecological adaptations. We revealed that specific miRNAs, especially Btab-miR-34 and Btab-miR-2944b, play critical roles in regulating these stages by repressing processes such as DNA replication and cuticle formation. Furthermore, novel miRNAs such as bta-miR-307a, bta-miR-352a, and bta-miR-107a have emerged as key regulators that direct pathways involved in detoxification and environmental sensing. Our analysis of transcriptional dynamics revealed that each life stage has a unique molecular blueprint. Eggs focus mainly on replication, whereas adults produce proteins related to detoxification and sensory functions. Proteomic data further confirmed that these molecular programs are functionally active during development. This network suggests that established miRNAs are crucial for maintaining developmental stability, whereas newer regulators support lineage-specific adaptations that improve survival. This research highlights the complex and finely tuned molecular mechanisms that regulate the growth and development of B. tabaci. These insights also offer valuable information that can help improve pest management strategies in agriculture.

ORGANISM(S): Bemisia Tabaci

SUBMITTER: Dr Murugan M

PROVIDER: PXD069799 | JPOST Repository | Thu Oct 23 00:00:00 BST 2025

REPOSITORIES: jPOST

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	TNAU-PRASH-ADULT-1.mzXML	Mzxml
	TNAU-PRASH-ADULT-2.mzXML	Mzxml
	TNAU-PRASH-ADULT-3.mzXML	Mzxml
	TNAU-PRASH-EGG-WITHOUTSTACK-1.mzXML	Mzxml
	TNAU-PRASH-EGG-WITHOUTSTACK-2.mzXML	Mzxml

Items per page:

1 - 5 of 20

Similar Datasets

Project description:Pochonia chlamydosporia (Goddard) Zare & Gams (Ascomycota, Sordariomycetes, Hypocreales, Pochoniaceae, Pochonia) is a nematophagous fungus with significant potential as a biocontrol agent against animal-parasitic nematode. However, the molecular and cellular mechanisms underlying its infection process remain poorly understood. This study aims to provide a comprehensive investigation of P. chlamydosporia infection dynamics in Parascaris equorum eggs using both microscopic and proteomic approaches. The infection was monitored at three distinct stages (early, middle, and late), with corresponding ultrastructural and molecular changes observed. Microscopic analysis using scanning electron microscopy (SEM), transmission electron microscopy (TEM), and light microscopy (LM) revealed the progressive invasion of P. chlamydosporia into nematode eggs. These observations provided detailed insights into the morphological changes in both fungal structures and nematode eggs, highlighting key infection stages such as fungal attachment, germination, and egg degradation. Furthermore, the observations confirmed the stages of fungal colonization, emphasizing the dynamic host-pathogen interaction at the macroscopic level. To complement these observations, a 4D-DIA-based quantitative proteomics approach was employed to analyze the exoproteomic changes in P. chlamydosporia during infection. A total of 410 differentially expressed proteins (DEPs) were identified across the three infection stages, with 313 proteins upregulated and 403 proteins downregulated. Gene Ontology (GO) enrichment analysis revealed that these DEPs are involved in critical biological processes, including cellular stress response, proteolysis, metabalic process, and hydrolase activity. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis further identified key infection-associated pathways, such as signal transduction, cell wall biosynthesis, energy metabolism, and host-pathogen interactions. These findings suggest that P. chlamydosporia employs a highly coordinated molecular strategy to adapt to and exploit its host. Quantitative PCR (qPCR) validation of key genes involved in signal transduction and immune evasion mechanisms further supported the molecular basis of P. chlamydosporia's parasitic behavior. These findings contribute to our understanding of fungal-nematode interactions and lay a solid foundation for the development of P. chlamydosporia as a sustainable tool for integrated pest management.

Dataset Information

A biphasic molecular program orchestrates ontogenetic transitions and ecological specialization in invasive whitefly, Bemisia tabaci

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets