Proteomics

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Proteomic profiling of nuclear fractions of SMARCA4 knockdown samples in HDMB03

ABSTRACT: SMARCA4 mutations are common in Medulloblastoma but not much is understood about its pathobiology. In this study we prepared SMARCA4 knockdown models using a Group 3 medulloblastoma cell line called HDMB03 and investigated the proteomic changes. Nuclear enrichment was performed using hypertonic and hypotonic buffers. The nuclear enriched fractions were analysed on LC-MS/MS.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Prof. Sanjeeva Srivastava 

PROVIDER: PXD070641 | JPOST Repository | Wed Mar 25 00:00:00 GMT 2026

REPOSITORIES: jPOST

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Publications

Quantitative DIA-MS Uncovers Functional Impact of SMARCA4 Knockdown in Group 3 Medulloblastoma.

Pai Medha Gayathri J MGJ   Singh Avinash A   Patra Sayan S   Narang Deepanshu D   Bapat Purna P   Bharambe Harish Shrikrishna HS   Shirsat Neelam N   Srivastava Sanjeeva S  

Journal of proteome research 20260326 5

Medulloblastoma, a pediatric brain tumor, frequently features chromatin modifier mutations, including SMARCA4 loss in the aggressive Group 3 subgroup. While SMARCA4 is considered a tumor suppressor, the functional impact of its loss on the oncogenic programs in Group 3 MB remains poorly understood. Using doxycycline-inducible shRNA constructs in HD-MB03 cells (a MYC-amplified Group MB model) to achieve SMARCA4 knockdown, we applied quantitative mass spectrometry to profile the resulting proteomi  ...[more]

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