Dataset Information
Dynamic protein acetylation changes in E. coli (carbon overflow)
Ontology highlight
ABSTRACT:
INSTRUMENT(S): TripleTOF 6600
ORGANISM(S): Escherichia Coli (ncbitaxon:562)
SUBMITTER: Bradford W. Gibson, Alan J. Wolfe
PROVIDER: MSV000079073 | MassIVE | Mon Mar 09 16:06:00 GMT 2015
SECONDARY ACCESSION(S): PXD001894
REPOSITORIES: MassIVE
Publications
Protein acetylation dynamics in response to carbon overflow in Escherichia coli.
Molecular microbiology 20150910 5
In Escherichia coli, acetylation of proteins at lysines depends largely on a non-enzymatic acetyl phosphate-dependent mechanism. To assess the functional significance of this post-translational modification, we first grew wild-type cells in buffered tryptone broth with glucose and monitored acetylation over time by immunochemistry. Most acetylation occurred in stationary phase and paralleled glucose consumption and acetate excretion, which began upon entry into stationary phase. Transcription of ...[more]
