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Shotgun analysis of nuclear proteins from mouse brain

ABSTRACT: Nuclei were isolated from the mouse brain. Proteins were extracted with addition of Bensonase and cleaned up by acetone precipitation. Proteis were resuspended in 50 mM ammonium bicarbonate and PPS Silent Surfactant. Samples were sonicated on ice to improve the solubility of proteins, then alkylated with IAA and digested with trypsin. Peptides were analyzed by LC-MS/MS using the UltiMate 3000RS nano-System coupled with microTOF_QII.

INSTRUMENT(S): micrOTOF-Q II

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Sylwia Kedracka-Krok

PROVIDER: MSV000079301 | MassIVE |

SECONDARY ACCESSION(S): PXD002872

REPOSITORIES: MassIVE

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Optimized procedure of extraction, purification and proteomic analysis of nuclear proteins from mouse brain.

Jankowska Urszula U Latosinska Agnieszka A Skupien-Rabian Bozena B Swiderska Bianka B Dziedzicka-Wasylewska Marta M Kedracka-Krok Sylwia S

Journal of neuroscience methods 20151214

<h4>Background</h4>The cell nucleus is a highly dynamic subcellular compartment performing crucial processes for functioning and administration of the cell. Proteomic analysis of isolated nuclear fraction enables in-depth insight into these processes leading to better understanding of physiological and pathological state of the brain. There is no universal method for nuclear proteome investigation and each biological material needs individual optimization. An additional difficulty is the large a ...[more]

PMID: 26698226

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