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Kinase peptide discovery in MIB enriched samples using 2m monolithic silica-C18 column

ABSTRACT: This dataset accompanies our publication "An optimized chromatographic strategy for multiplexing in parallel reaction monitoring mass spectrometry: Insights from quantitation of activated kinases" in Molecular & Cellular Proteomics, 2016 (https://www.ncbi.nlm.nih.gov/pubmed/27940637). The submitted data were used to construct a reference spectral library for kinase detection.

INSTRUMENT(S): Q Exactive

SUBMITTER: Alma L. Burlingame

PROVIDER: MSV000079644 | MassIVE | Sun Apr 10 16:41:00 BST 2016

SECONDARY ACCESSION(S): PXD003934

REPOSITORIES: MassIVE

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			Action	DRS
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	P20140109-09.mzXML	Mzxml
	P20140109-11.mzXML	Mzxml
	P20140109-13.mzXML	Mzxml
	P20140109-15.mzXML	Mzxml

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Publications

An Optimized Chromatographic Strategy for Multiplexing In Parallel Reaction Monitoring Mass Spectrometry: Insights from Quantitation of Activated Kinases.

Urisman Anatoly A Levin Rebecca S RS Gordan John D JD Webber James T JT Hernandez Hilda H Ishihama Yasushi Y Shokat Kevan M KM Burlingame Alma L AL

Molecular & cellular proteomics : MCP 20161211 2

Reliable quantitation of protein abundances in defined sets of cellular proteins is critical to numerous biological applications. Traditional immunodetection-based methods are limited by the quality and availability of specific antibodies, especially for site-specific post-translational modifications. Targeted proteomic methods, including the recently developed parallel reaction monitoring (PRM) mass spectrometry, have enabled accurate quantitative measurements of up to a few hundred specific ta ...[more]

PMID: 27940637

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